Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS
Previous findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of pol...
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doaj-070c00deec5444949ee6214f924133e72020-11-25T01:30:46ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-08-01168183961841110.3390/ijms160818396ijms160818396Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MSIsabel Borrás-Linares0María Herranz-López1Enrique Barrajón-Catalán2David Arráez-Román3Isabel Gonzálezlvarez4Marival Bermejo5Alberto Fernández Gutiérrez6Vicente Micol7Antonio Segura-Carretero8Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, SpainInstituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, SpainInstituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, SpainPharmacokinetics and Pharmaceutical Technology Area, Engineering Department, Universidad Miguel Hernández, San Juan de Alicante, 03550 Alicante, SpainPharmacokinetics and Pharmaceutical Technology Area, Engineering Department, Universidad Miguel Hernández, San Juan de Alicante, 03550 Alicante, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, SpainInstituto de Biología Molecular y Celular (IBMC), Miguel Hernández University, Elche, 03202 Alicante, SpainDepartment of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda Fuentenueva s/n, 18071 Granada, SpainPrevious findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS), free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine) were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS), and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols.http://www.mdpi.com/1422-0067/16/8/18396Hibiscus sabdariffaphenolic compoundsflavonoidsCaco-2 cellsUHPLC-ESI-UHR-Qq-TOF-MS |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Isabel Borrás-Linares María Herranz-López Enrique Barrajón-Catalán David Arráez-Román Isabel Gonzálezlvarez Marival Bermejo Alberto Fernández Gutiérrez Vicente Micol Antonio Segura-Carretero |
spellingShingle |
Isabel Borrás-Linares María Herranz-López Enrique Barrajón-Catalán David Arráez-Román Isabel Gonzálezlvarez Marival Bermejo Alberto Fernández Gutiérrez Vicente Micol Antonio Segura-Carretero Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS International Journal of Molecular Sciences Hibiscus sabdariffa phenolic compounds flavonoids Caco-2 cells UHPLC-ESI-UHR-Qq-TOF-MS |
author_facet |
Isabel Borrás-Linares María Herranz-López Enrique Barrajón-Catalán David Arráez-Román Isabel Gonzálezlvarez Marival Bermejo Alberto Fernández Gutiérrez Vicente Micol Antonio Segura-Carretero |
author_sort |
Isabel Borrás-Linares |
title |
Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS |
title_short |
Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS |
title_full |
Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS |
title_fullStr |
Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS |
title_full_unstemmed |
Permeability Study of Polyphenols Derived from a Phenolic-Enriched Hibiscus sabdariffa Extract by UHPLC-ESI-UHR-Qq-TOF-MS |
title_sort |
permeability study of polyphenols derived from a phenolic-enriched hibiscus sabdariffa extract by uhplc-esi-uhr-qq-tof-ms |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2015-08-01 |
description |
Previous findings on the capacity of Hibiscus sabdariffa (HS) polyphenols to ameliorate metabolic disturbances justify the necessity of studies oriented to find the potential metabolites responsible for such an effect. The present study examined the intestinal epithelial membrane permeability of polyphenols present in a phenolic-enriched Hibiscus sabdariffa extract (PEHS), free and encapsulated, using the Caco-2 cell line. Additionally, selected polyphenols (quercetin, quercetin-3-glucoside, quercetin-3-glucuronide, and N-feruloyltyramine) were also studied in the same absorption model. The powerful analytical platform used ultra-high-performance liquid chromatography coupled with ultra-high-resolution quadrupole time-of-flight mass spectrometry (UHPLC-ESI-UHR-Qq-TOF-MS), and enabled the characterization of seven new compounds in PEHS. In the permeation study, only a few compounds were able to cross the cell monolayer and the permeability was lower when the extract was in an encapsulated form. Pure compounds showed a moderate absorption in all cases. Nevertheless, these preliminary results may need further research to understand the complete absorption mechanism of Hibiscus polyphenols. |
topic |
Hibiscus sabdariffa phenolic compounds flavonoids Caco-2 cells UHPLC-ESI-UHR-Qq-TOF-MS |
url |
http://www.mdpi.com/1422-0067/16/8/18396 |
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