High-level soluble expression of phospholipase D from Streptomyces chromofuscus in Escherichia coli by combinatorial optimization

High-level soluble expression of phospholipase D from Streptomyces chromofuscus in Escherichia coli by combinatorial optimization

Background: Phospholipase D (PLD) is used as the biocatalyst for phosphatidylserine (PS) production. In general, PLD was expressed in insoluble form in Escherichia coli. High-level soluble expression of PLD with high activity in E. coli is very important for industrial production of PLD. Results: St...

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Bibliographic Details
Main Authors: Rong Wu, Jun Cao, Feixiang Liu, Meng Yang, Erzheng Su
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:Electronic Journal of Biotechnology
Subjects:
Streptomyces chromofuscus
Phospholipase D (PLD)
Soluble expression
Lactose induction
Combinatorial optimization
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345820300622

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http://www.sciencedirect.com/science/article/pii/S0717345820300622

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