Phage-assisted continuous evolution of proteases with altered substrate specificity

Phage-assisted continuous evolution of proteases with altered substrate specificity

Proteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence specificities.

Bibliographic Details
Main Authors: Michael S. Packer, Holly A. Rees, David R. Liu
Format: Article
Language:English
Published: Nature Publishing Group 2017-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-01055-9
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spelling doaj-08222d2b844046fe8d313002c7e42c082021-05-11T07:12:28ZengNature Publishing GroupNature Communications2041-17232017-10-018111110.1038/s41467-017-01055-9Phage-assisted continuous evolution of proteases with altered substrate specificityMichael S. Packer0Holly A. Rees1David R. Liu2Department of Chemistry and Chemical Biology, Harvard UniversityDepartment of Chemistry and Chemical Biology, Harvard UniversityDepartment of Chemistry and Chemical Biology, Harvard UniversityProteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence specificities.https://doi.org/10.1038/s41467-017-01055-9
collection DOAJ
language English
format Article
sources DOAJ
author Michael S. Packer
Holly A. Rees
David R. Liu
spellingShingle Michael S. Packer
Holly A. Rees
David R. Liu
Phage-assisted continuous evolution of proteases with altered substrate specificity
Nature Communications
author_facet Michael S. Packer
Holly A. Rees
David R. Liu
author_sort Michael S. Packer
title Phage-assisted continuous evolution of proteases with altered substrate specificity
title_short Phage-assisted continuous evolution of proteases with altered substrate specificity
title_full Phage-assisted continuous evolution of proteases with altered substrate specificity
title_fullStr Phage-assisted continuous evolution of proteases with altered substrate specificity
title_full_unstemmed Phage-assisted continuous evolution of proteases with altered substrate specificity
title_sort phage-assisted continuous evolution of proteases with altered substrate specificity
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-10-01
description Proteases are promising therapeutics to treat diseases such as hemophilia which are due to endogenous protease deficiency. Here the authors use phage-assisted continuous evolution to evolve a variant TEV protease with altered target peptide sequence specificities.
url https://doi.org/10.1038/s41467-017-01055-9
work_keys_str_mv AT michaelspacker phageassistedcontinuousevolutionofproteaseswithalteredsubstratespecificity
AT hollyarees phageassistedcontinuousevolutionofproteaseswithalteredsubstratespecificity
AT davidrliu phageassistedcontinuousevolutionofproteaseswithalteredsubstratespecificity
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