Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris

Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57 in Escherichia coli DH5α, but only found the...

Full description

Bibliographic Details
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2017-07-01
Series:Microbiology Indonesia
Subjects:
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/550