Cloning of Synthetic Lipase Gene from Rhizomucor miehei with Original Signal Peptide in Pichia pastoris
Lipases (EC 3.1.1.3) are classified as hydrolases that hydrolyze lipids. These enzymes have potential application in biotechnology and industrial process. In previous study we have cloned the synthetic Rhizomucor miehei lipase gene using the vector pUC57 in Escherichia coli DH5α, but only found the...
Format: | Article |
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Language: | English |
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Indonesian Society for Microbiology
2017-07-01
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Series: | Microbiology Indonesia |
Subjects: | |
Online Access: | https://jurnal.permi.or.id/index.php/mionline/article/view/550 |