Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs
Understanding the cellular behavioral mechanisms underlying memory formation and maintenance in human induced pluripotent stem cell (hiPSC) culture provides key strategies for achieving stability and robustness of cell differentiation. Here, we show that changes in cell behavior-driven epigenetic me...
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doaj-087fae3087e843bc885c26a0e42977482021-09-15T04:20:57ZengElsevierStem Cell Research1873-50612021-10-0156102534Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCsMee-Hae Kim0Naruchit Thanuthanakhun1Shun Fujimoto2Masahiro Kino-oka3Corresponding author.; Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDepartment of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanUnderstanding the cellular behavioral mechanisms underlying memory formation and maintenance in human induced pluripotent stem cell (hiPSC) culture provides key strategies for achieving stability and robustness of cell differentiation. Here, we show that changes in cell behavior-driven epigenetic memory of hiPSC cultures alter their pluripotent state and subsequent differentiation. Interestingly, pluripotency-associated genes were activated during the entire cell growth phases along with increased active modifications and decreased repressive modifications. This memory effect can last several days in the long-term stationary phase and was sustained in the aspect of cell behavioral changes after subculture. Further, changes in growth-related cell behavior were found to induce nucleoskeletal reorganization and active versus repressive modifications, thereby enabling hiPSCs to change their differentiation potential. Overall, we discuss the cell behavior-driven epigenetic memory induced by the culture environment, and the effect of previous memory on cell lineage specification in the process of hiPSC differentiation.http://www.sciencedirect.com/science/article/pii/S1873506121003810Human induced pluripotent stem cellsProliferationDifferentiation potentialMechanical effectsBehavioral epigenetic memory |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mee-Hae Kim Naruchit Thanuthanakhun Shun Fujimoto Masahiro Kino-oka |
spellingShingle |
Mee-Hae Kim Naruchit Thanuthanakhun Shun Fujimoto Masahiro Kino-oka Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs Stem Cell Research Human induced pluripotent stem cells Proliferation Differentiation potential Mechanical effects Behavioral epigenetic memory |
author_facet |
Mee-Hae Kim Naruchit Thanuthanakhun Shun Fujimoto Masahiro Kino-oka |
author_sort |
Mee-Hae Kim |
title |
Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs |
title_short |
Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs |
title_full |
Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs |
title_fullStr |
Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs |
title_full_unstemmed |
Effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human iPSCs |
title_sort |
effect of initial seeding density on cell behavior-driven epigenetic memory and preferential lineage differentiation of human ipscs |
publisher |
Elsevier |
series |
Stem Cell Research |
issn |
1873-5061 |
publishDate |
2021-10-01 |
description |
Understanding the cellular behavioral mechanisms underlying memory formation and maintenance in human induced pluripotent stem cell (hiPSC) culture provides key strategies for achieving stability and robustness of cell differentiation. Here, we show that changes in cell behavior-driven epigenetic memory of hiPSC cultures alter their pluripotent state and subsequent differentiation. Interestingly, pluripotency-associated genes were activated during the entire cell growth phases along with increased active modifications and decreased repressive modifications. This memory effect can last several days in the long-term stationary phase and was sustained in the aspect of cell behavioral changes after subculture. Further, changes in growth-related cell behavior were found to induce nucleoskeletal reorganization and active versus repressive modifications, thereby enabling hiPSCs to change their differentiation potential. Overall, we discuss the cell behavior-driven epigenetic memory induced by the culture environment, and the effect of previous memory on cell lineage specification in the process of hiPSC differentiation. |
topic |
Human induced pluripotent stem cells Proliferation Differentiation potential Mechanical effects Behavioral epigenetic memory |
url |
http://www.sciencedirect.com/science/article/pii/S1873506121003810 |
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1717379374453358592 |