Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

Summary: Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated c...

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Bibliographic Details
Main Authors: José Ángel Nicolás-Ávila, María Sánchez-Diaz, Andrés Hidalgo
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302732