Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
Summary: Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated c...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2021-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720302732 |