Ex vivo assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells

Ex vivo assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells

Summary: This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating...

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Bibliographic Details
Main Authors: Katrin Anne Becker, Alexander Carpinteiro, Markus Hoffmann, Stefan Pöhlmann, Johannes Kornhuber, Erich Gulbins
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell isolation
Cell-based assays
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721000630

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http://www.sciencedirect.com/science/article/pii/S2666166721000630

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