Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction.

Development of Real-Time PCR Methods for the Detection of Bacterial Meningitis Pathogens without DNA Extraction.

Neisseria meningitidis (Nm), Haemophilus influenzae (Hi), and Streptococcus pneumoniae (Sp) are the lead causes of bacterial meningitis. Detection of these pathogens from clinical specimens using traditional real-time PCR (rt-PCR) requires DNA extraction to remove the PCR inhibitors prior to testing...

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Bibliographic Details
Main Authors: Jeni Vuong, Jean-Marc Collard, Melissa J Whaley, Issaka Bassira, Issaka Seidou, Seydou Diarra, Rasmata T Ouédraogo, Dinanibè Kambiré, Thomas H Taylor, Claudio Sacchi, Leonard W Mayer, Xin Wang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4735509?pdf=render

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http://europepmc.org/articles/PMC4735509?pdf=render

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