Development of a scalable and robust AEX method for enriched rAAV preparations in genome-containing VCs of serotypes 5, 6, 8, and 9

Development of a scalable and robust AEX method for enriched rAAV preparations in genome-containing VCs of serotypes 5, 6, 8, and 9

Removal of empty capsids from adeno-associated virus (AAV) manufacturing lots remains a critical step in the downstream processing of AAV clinical-grade batches. Because of similar physico-chemical characteristics, the AAV capsid populations totally lacking or containing partial viral DNA are diffic...

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Bibliographic Details
Main Authors: Pranav R.H. Joshi, Alice Bernier, Pablo D. Moço, Joseph Schrag, Parminder S. Chahal, Amine Kamen
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
AAV vectors
AAV serotype-5, 6, 8, 9
separation of genome containing/empty AAV capsids
anion-exchange chromatography
downstream processing
scale-up
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050121000565

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