Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells
Abstract Background Phospholipid phosphatase 4 (PPAPDC1A or PLPP4) has been demonstrated to be involved in the malignant process of many cancers. The purpose of this study was to investigate the clinical significance and biological roles of PLPP4 in lung carcinoma. Methods PLPP4 expression was exami...
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BMC
2017-08-01
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Series: | Molecular Cancer |
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Online Access: | http://link.springer.com/article/10.1186/s12943-017-0717-5 |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xin Zhang Lan Zhang Bihua Lin Xingxing Chai Ronggang Li Yuehua Liao Xinghui Deng Qiongru Liu Wenli Yang Yubo Cai Wei Zhou Zhichao Lin Wenhai Huang Meigong Zhong Fangyong Lei Jinhua Wu Shuaishuai Yu Xiaoping Li Shangren Li Yueyue Li Jincheng Zeng Wansheng Long Dong Ren Yanming Huang |
spellingShingle |
Xin Zhang Lan Zhang Bihua Lin Xingxing Chai Ronggang Li Yuehua Liao Xinghui Deng Qiongru Liu Wenli Yang Yubo Cai Wei Zhou Zhichao Lin Wenhai Huang Meigong Zhong Fangyong Lei Jinhua Wu Shuaishuai Yu Xiaoping Li Shangren Li Yueyue Li Jincheng Zeng Wansheng Long Dong Ren Yanming Huang Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells Molecular Cancer PLPP4 Proliferation Cell cycle Tumorigenesis Ca2+-permeable cationic channel Therapeutic target |
author_facet |
Xin Zhang Lan Zhang Bihua Lin Xingxing Chai Ronggang Li Yuehua Liao Xinghui Deng Qiongru Liu Wenli Yang Yubo Cai Wei Zhou Zhichao Lin Wenhai Huang Meigong Zhong Fangyong Lei Jinhua Wu Shuaishuai Yu Xiaoping Li Shangren Li Yueyue Li Jincheng Zeng Wansheng Long Dong Ren Yanming Huang |
author_sort |
Xin Zhang |
title |
Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells |
title_short |
Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells |
title_full |
Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells |
title_fullStr |
Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells |
title_full_unstemmed |
Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cells |
title_sort |
phospholipid phosphatase 4 promotes proliferation and tumorigenesis, and activates ca2+-permeable cationic channel in lung carcinoma cells |
publisher |
BMC |
series |
Molecular Cancer |
issn |
1476-4598 |
publishDate |
2017-08-01 |
description |
Abstract Background Phospholipid phosphatase 4 (PPAPDC1A or PLPP4) has been demonstrated to be involved in the malignant process of many cancers. The purpose of this study was to investigate the clinical significance and biological roles of PLPP4 in lung carcinoma. Methods PLPP4 expression was examined in 8 paired lung carcinoma tissues by real-time PCR and in 265 lung carcinoma tissues by immunohistochemistry (IHC). Statistical analysis was performed to evaluate the clinical correlation between PLPP4 expression and clinicopathological features and survival in lung carcinoma patients. In vitro and in vivo assays were performed to assess the biological roles of PLPP4 in lung carcinoma. Fluorescence-activated cell sorting, Western blotting and luciferase assays were used to identify the underlying pathway through which PLPP4 silencing mediates biological roles in lung carcinoma. Results PLPP4 is differentially elevated in lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SQC) tissues. Statistical analysis demonstrated that high expression of PLPP4 significantly and positively correlated with clinicopathological features, including pathological grade, T category and stage, and poor overall and progression-free survival in lung carcinoma patients. Silencing PLPP4 inhibits proliferation and cell cycle progression in vitro and tumorigenesis in vivo in lung carcinoma cells. Our results further reveal that PLPP4 silencing inhibits Ca2+-permeable cationic channel, suggesting that downregulation of PLPP4 inhibits proliferation and tumorigenesis in lung carcinoma cells via reducing the influx of intracellular Ca2+. Conclusion Our results indicate that PLPP4 may hold promise as a novel marker for the diagnosis of lung carcinoma and as a potential therapeutic target to facilitate the development of novel treatment for lung carcinoma. |
topic |
PLPP4 Proliferation Cell cycle Tumorigenesis Ca2+-permeable cationic channel Therapeutic target |
url |
http://link.springer.com/article/10.1186/s12943-017-0717-5 |
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doaj-08efc75b4e234adf9d1575d378f5d3802020-11-24T21:11:06ZengBMCMolecular Cancer1476-45982017-08-0116111410.1186/s12943-017-0717-5Phospholipid Phosphatase 4 promotes proliferation and tumorigenesis, and activates Ca2+-permeable Cationic Channel in lung carcinoma cellsXin Zhang0Lan Zhang1Bihua Lin2Xingxing Chai3Ronggang Li4Yuehua Liao5Xinghui Deng6Qiongru Liu7Wenli Yang8Yubo Cai9Wei Zhou10Zhichao Lin11Wenhai Huang12Meigong Zhong13Fangyong Lei14Jinhua Wu15Shuaishuai Yu16Xiaoping Li17Shangren Li18Yueyue Li19Jincheng Zeng20Wansheng Long21Dong Ren22Yanming Huang23Clinical Experimental Center, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityGuangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Department of Obstetrics and Gynecology, Sun Yat-Sen Memorial Hospital, Sun Yat-sen UniversityDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical UniversityLaboratory Animal Center, Guangdong Medical UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pathology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Thoracic Surgery, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Thoracic Surgery, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Pharmacy, Jiangmen Maternity and Child Health Care HospitalDepartment of Oncology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Clinical Laboratory, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Clinical Laboratory, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of General Surgery, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of General Surgery, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDepartment of Radiology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical UniversityDepartment of Radiology, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityDongguan Key Laboratory of Medical Bioactive Molecular Developmental and Translational Research, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical UniversityClinical Experimental Center, Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen UniversityAbstract Background Phospholipid phosphatase 4 (PPAPDC1A or PLPP4) has been demonstrated to be involved in the malignant process of many cancers. The purpose of this study was to investigate the clinical significance and biological roles of PLPP4 in lung carcinoma. Methods PLPP4 expression was examined in 8 paired lung carcinoma tissues by real-time PCR and in 265 lung carcinoma tissues by immunohistochemistry (IHC). Statistical analysis was performed to evaluate the clinical correlation between PLPP4 expression and clinicopathological features and survival in lung carcinoma patients. In vitro and in vivo assays were performed to assess the biological roles of PLPP4 in lung carcinoma. Fluorescence-activated cell sorting, Western blotting and luciferase assays were used to identify the underlying pathway through which PLPP4 silencing mediates biological roles in lung carcinoma. Results PLPP4 is differentially elevated in lung adenocarcinoma (ADC) and lung squamous cell carcinoma (SQC) tissues. Statistical analysis demonstrated that high expression of PLPP4 significantly and positively correlated with clinicopathological features, including pathological grade, T category and stage, and poor overall and progression-free survival in lung carcinoma patients. Silencing PLPP4 inhibits proliferation and cell cycle progression in vitro and tumorigenesis in vivo in lung carcinoma cells. Our results further reveal that PLPP4 silencing inhibits Ca2+-permeable cationic channel, suggesting that downregulation of PLPP4 inhibits proliferation and tumorigenesis in lung carcinoma cells via reducing the influx of intracellular Ca2+. Conclusion Our results indicate that PLPP4 may hold promise as a novel marker for the diagnosis of lung carcinoma and as a potential therapeutic target to facilitate the development of novel treatment for lung carcinoma.http://link.springer.com/article/10.1186/s12943-017-0717-5PLPP4ProliferationCell cycleTumorigenesisCa2+-permeable cationic channelTherapeutic target |