Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor

Abstract Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein, we introduce two strategies to impro...

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Bibliographic Details
Main Authors: Soo-Ji Park, Tae Yeong Jeong, Seung Kyun Shin, Da Eun Yoon, Soo-Yeon Lim, Sol Pin Kim, Jungmin Choi, Hyunji Lee, Jeong-Im Hong, Jinhee Ahn, Je Kyung Seong, Kyoungmi Kim
Format: Article
Language:English
Published: BMC 2021-06-01
Series:Genome Biology
Subjects:
Prime editor
Igf2
Adamts20
Mouse cells and embryos
Germline transmission
Dwarf phenotype
Online Access:https://doi.org/10.1186/s13059-021-02389-w

Internet

https://doi.org/10.1186/s13059-021-02389-w

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