A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells

A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells

Single-domain antibodies have emerged as highly versatile nanoprobes for advanced cellular imaging. For real-time visualization of endogenous antigens, fluorescently labelled nanobodies (chromobodies, CBs) are introduced as DNA-encoded expression constructs in living cells. Commonly, CB expression i...

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Bibliographic Details
Main Authors: Bettina-Maria Keller, Julia Maier, Melissa Weldle, Soeren Segan, Bjoern Traenkle, Ulrich Rothbauer
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Antibodies
Subjects:
nanobodies
chromobodies
live-cell imaging
compound screening
cellular models
Online Access:http://www.mdpi.com/2073-4468/8/1/10

Internet

http://www.mdpi.com/2073-4468/8/1/10

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