Establishment and Identification of Chinese Hamster Ovary Cell Lines with Stable Expression of Soluble CD40 Ligands

Establishment and Identification of Chinese Hamster Ovary Cell Lines with Stable Expression of Soluble CD40 Ligands

Objective: To establish the Chinese Hamster Ovary (CHO) cell lines with stable expression of soluble CD40 ligands (sCD40L). Methods: Recombinant plasmid pIRES2-EGFP-sCD40L, enzyme digestion and sequencing identification were obtained by cloning sCD40L coding sequences into eukaryotic expression ve...

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Bibliographic Details
Main Authors: JIANG Hua-wei, YANG Quan-liang, LIU Yong-ping, LING Yang
Format: Article
Language:English
Published: Third Party Medicine International Publishing Group Co. Limited 2014-09-01
Series:Journal of International Translational Medicine
Subjects:
Soluble CD40 ligand; Green fluorescent protein; Apoptosis; Fatty acid synthase
Online Access:http://www.jitm.hk/EN/10.11910/2227-6394.2014.02.03.05

Internet

http://www.jitm.hk/EN/10.11910/2227-6394.2014.02.03.05

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