Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA

Pre-digest of unprotected DNA by Benzonase improves the representation of living skin bacteria and efficiently depletes host DNA

Abstract Background The identification of microbiota based on next-generation sequencing (NGS) of extracted DNA has drastically improved our understanding of the role of microbial communities in health and disease. However, DNA-based microbiome analysis cannot per se differentiate between living and...

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Bibliographic Details
Main Authors: Yacine Amar, Ilias Lagkouvardos, Rafaela L. Silva, Oluwaseun Ayodeji Ishola, Bärbel U. Foesel, Susanne Kublik, Anne Schöler, Sebastian Niedermeier, Rachela Bleuel, Alexander Zink, Klaus Neuhaus, Michael Schloter, Tilo Biedermann, Martin Köberle
Format: Article
Language:English
Published: BMC 2021-05-01
Series:Microbiome
Subjects:
Benzonase
DNA extraction
Next-generation sequencing
Skin microbiome
16S rRNA
Diversity
Online Access:https://doi.org/10.1186/s40168-021-01067-0

Internet

https://doi.org/10.1186/s40168-021-01067-0

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