The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb
Microglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication...
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doaj-0970523c2f0d422a9f39263df597df682021-05-05T14:01:00ZengeLife Sciences Publications LtdeLife2050-084X2017-12-01610.7554/eLife.30809The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulbRonen Reshef0https://orcid.org/0000-0003-3970-6687Elena Kudryavitskaya1Haran Shani-Narkiss2Batya Isaacson3Neta Rimmerman4Adi Mizrahi5Raz Yirmiya6https://orcid.org/0000-0002-4009-7316Department of Psychology, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Neurobiology, Institute for Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Neurobiology, Institute for Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Immunology and Cancer Research, The Lautenberg Center for General and Tumor Immunology, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Psychology, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Neurobiology, Institute for Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University of Jerusalem, Jerusalem, IsraelDepartment of Psychology, The Hebrew University of Jerusalem, Jerusalem, IsraelMicroglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient (Cx3cr1−/−) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In Cx3cr1−/− mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between Cx3cr1-deficient microglia and abGCs' dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry.https://elifesciences.org/articles/30809adult neruogenesissynapsesmicrogliaolfactory bulbspinesdevelopment |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ronen Reshef Elena Kudryavitskaya Haran Shani-Narkiss Batya Isaacson Neta Rimmerman Adi Mizrahi Raz Yirmiya |
spellingShingle |
Ronen Reshef Elena Kudryavitskaya Haran Shani-Narkiss Batya Isaacson Neta Rimmerman Adi Mizrahi Raz Yirmiya The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb eLife adult neruogenesis synapses microglia olfactory bulb spines development |
author_facet |
Ronen Reshef Elena Kudryavitskaya Haran Shani-Narkiss Batya Isaacson Neta Rimmerman Adi Mizrahi Raz Yirmiya |
author_sort |
Ronen Reshef |
title |
The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb |
title_short |
The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb |
title_full |
The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb |
title_fullStr |
The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb |
title_full_unstemmed |
The role of microglia and their CX3CR1 signaling in adult neurogenesis in the olfactory bulb |
title_sort |
role of microglia and their cx3cr1 signaling in adult neurogenesis in the olfactory bulb |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2017-12-01 |
description |
Microglia play important roles in perinatal neuro- and synapto-genesis. To test the role of microglia in these processes during adulthood, we examined the effects of microglia depletion, via treatment of mice with the CSF-1 receptor antagonist PLX5622, and abrogated neuronal-microglial communication in CX3C receptor-1 deficient (Cx3cr1−/−) mice. Microglia depletion significantly lowered spine density in young (developing) but not mature adult-born-granule-cells (abGCs) in the olfactory bulb. Two-photon time-lapse imaging indicated that microglia depletion reduced spine formation and elimination. Functionally, odor-evoked responses of mitral cells, which are normally inhibited by abGCs, were increased in microglia-depleted mice. In Cx3cr1−/− mice, abGCs exhibited reduced spine density, dynamics and size, concomitantly with reduced contacts between Cx3cr1-deficient microglia and abGCs' dendritic shafts, along with increased proportion of microglia-contacted spines. Thus, during adult neurogenesis, microglia regulate the elimination (pruning), formation, and maintenance of synapses on newborn neurons, contributing to the functional integrity of the olfactory bulb circuitry. |
topic |
adult neruogenesis synapses microglia olfactory bulb spines development |
url |
https://elifesciences.org/articles/30809 |
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