Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians
The genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APO...
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Associação Brasileira de Divulgação Científica
2003-07-01
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doaj-09958d64d9e34990a1fda1ae4e12142b2020-11-24T22:43:30ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2003-07-0136791992310.1590/S0100-879X2003000700013Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in BraziliansD.R.S. SouzaM.R. De GodoyJ. HottaE.H. TajaraA.C. BrandãoS. Pinheiro JúniorW.A. TognolaJ.E. Dos SantosThe genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APOE gene in late-onset Alzheimer's disease (N = 68), other late-life dementias (N = 39), and in cognitively normal controls (N = 58) was determined, as also was the risk for Alzheimer's disease associated with the epsilon4 allele. Peripheral blood samples were obtained from a total of 165 individuals living in Brazil aged 65-82 years. Genomic DNA was amplified by the polymerase chain reaction and the products were digested with HhaI restriction enzyme. APOE epsilon2 frequency was considerably lower in the Alzheimer's disease group (1%), and the epsilon3 allele and epsilon3/epsilon3 genotype frequencies were higher in the controls (84 and 72%, respectively) as were the epsilon4 allele and epsilon3/epsilon4 genotype frequencies in Alzheimer's disease (25 and 41%, respectively). The higher frequency of the epsilon4 allele in Alzheimer's disease confirmed its role as a risk factor, while epsilon2 provided a weak protection against development of the disease. However, in view of the unexpectedly low frequency of the epsilon4 allele, additional analyses in a more varied Brazilian sample are needed to clarify the real contribution of apolipoprotein E to the development of Alzheimer's disease in this population.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700013Alzheimer's diseaseVascular dementiaDementiaApolipoprotein EAgingGenetic polymorphisms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
D.R.S. Souza M.R. De Godoy J. Hotta E.H. Tajara A.C. Brandão S. Pinheiro Júnior W.A. Tognola J.E. Dos Santos |
spellingShingle |
D.R.S. Souza M.R. De Godoy J. Hotta E.H. Tajara A.C. Brandão S. Pinheiro Júnior W.A. Tognola J.E. Dos Santos Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians Brazilian Journal of Medical and Biological Research Alzheimer's disease Vascular dementia Dementia Apolipoprotein E Aging Genetic polymorphisms |
author_facet |
D.R.S. Souza M.R. De Godoy J. Hotta E.H. Tajara A.C. Brandão S. Pinheiro Júnior W.A. Tognola J.E. Dos Santos |
author_sort |
D.R.S. Souza |
title |
Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians |
title_short |
Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians |
title_full |
Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians |
title_fullStr |
Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians |
title_full_unstemmed |
Association of apolipoprotein E polymorphism in late-onset Alzheimer's disease and vascular dementia in Brazilians |
title_sort |
association of apolipoprotein e polymorphism in late-onset alzheimer's disease and vascular dementia in brazilians |
publisher |
Associação Brasileira de Divulgação Científica |
series |
Brazilian Journal of Medical and Biological Research |
issn |
0100-879X 1414-431X |
publishDate |
2003-07-01 |
description |
The genetic basis for dementias is complex. A common polymorphism in the apolipoprotein E (APOE) gene is considered to be the major risk factor in families with sporadic and late-onset Alzheimer's disease as well as in the general population. The distribution of alleles and genotypes of the APOE gene in late-onset Alzheimer's disease (N = 68), other late-life dementias (N = 39), and in cognitively normal controls (N = 58) was determined, as also was the risk for Alzheimer's disease associated with the epsilon4 allele. Peripheral blood samples were obtained from a total of 165 individuals living in Brazil aged 65-82 years. Genomic DNA was amplified by the polymerase chain reaction and the products were digested with HhaI restriction enzyme. APOE epsilon2 frequency was considerably lower in the Alzheimer's disease group (1%), and the epsilon3 allele and epsilon3/epsilon3 genotype frequencies were higher in the controls (84 and 72%, respectively) as were the epsilon4 allele and epsilon3/epsilon4 genotype frequencies in Alzheimer's disease (25 and 41%, respectively). The higher frequency of the epsilon4 allele in Alzheimer's disease confirmed its role as a risk factor, while epsilon2 provided a weak protection against development of the disease. However, in view of the unexpectedly low frequency of the epsilon4 allele, additional analyses in a more varied Brazilian sample are needed to clarify the real contribution of apolipoprotein E to the development of Alzheimer's disease in this population. |
topic |
Alzheimer's disease Vascular dementia Dementia Apolipoprotein E Aging Genetic polymorphisms |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000700013 |
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