Marine Cyclotripeptide X-13 Promotes Angiogenesis in Zebrafish and Human Endothelial Cells via PI3K/Akt/eNOS Signaling Pathways

• Main Authors: Zhong Pei, Hong-Shuo Sun, Li-Ping Wang, Ji-Yan Pang, Jing-Song Ou, Jin-Sheng Zeng, Ru-Xun Huang, Guang-Lei Wang, Yong-Cheng Lin, Jing Li, Cheng-Hui Ye, Feng-Juan Su, Xiao-Li Yao, Zhong-Liang Xu, Xi-Lin Lu
• Format: Article
• Language: English
• Published: MDPI AG 2012-06-01
• Series: Marine Drugs
• Subjects: angiogenesis; marine cyclotripeptide; zebrafish; endothelial nitric oxide synthase; xyloallenoide A
• Online Access: http://www.mdpi.com/1660-3397/10/6/1307

Cyclotripeptide X-13 is a core of novel marine compound xyloallenoide A isolated from mangrove fungus <em>Xylaria</em> sp. (no. 2508). We found that X-13 dose-dependently induced angiogenesis in zebrafish embryos and in human endothelial cells, which was accompanied by increased phosphorylation of eNOS and Akt and NO release. Inhibition of PI3K/Akt/eNOS by LY294002 or l-NAME suppressed X-13-induced angiogenesis. The present work demonstrates that X-13 promotes angiogenesis via PI3K/Akt/eNOS pathways.