BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B

BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B

Abstract Background Base Editing is a precise genome editing method that uses a deaminase-Cas9 fusion protein to mutate cytidine to thymidine in target DNA in situ without the generation of a double-strand break. However, the efficient enrichment of genetically modified cells using this technique is...

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Bibliographic Details
Main Authors: Matthew A. Coelho, Songyuan Li, Luna Simona Pane, Mike Firth, Giovanni Ciotta, Jonathan D. Wrigley, Maria Emanuela Cuomo, Marcello Maresca, Benjamin J. M. Taylor
Format: Article
Language:English
Published: BMC 2018-12-01
Series:BMC Biology
Subjects:
Base editing
Fluorescent reporter
CRISPR/Cas9
APOBEC
Gene editing
Online Access:http://link.springer.com/article/10.1186/s12915-018-0617-1

Internet

http://link.springer.com/article/10.1186/s12915-018-0617-1

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