Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard

Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard

Abstract Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here, we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental sample using a synthetic standard in minus...

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Bibliographic Details
Main Authors: Olivier Zemb, Caroline S. Achard, Jerome Hamelin, Marie‐Léa De Almeida, Béatrice Gabinaud, Laurent Cauquil, Lisanne M.G. Verschuren, Jean‐Jacques Godon
Format: Article
Language:English
Published: Wiley 2020-03-01
Series:MicrobiologyOpen
Subjects:
16S rRNA gene
absolute count data
metabarcoding
microbiome
normalization
spike‐in
Online Access:https://doi.org/10.1002/mbo3.977

Internet

https://doi.org/10.1002/mbo3.977

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