Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells

Technical approaches to reduce interference of Fetal calf serum derived RNA in the analysis of extracellular vesicle RNA from cultured cells

Foetal calf serum (FCS) is a common supplement of cell culture medium and a known source of contaminating extracellular vesicles (EV) containing RNA. Because of a high degree of sequence similarity among homologous non-coding RNAs of mammalian species, residual FCS-RNA in culture medium may interfer...

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Bibliographic Details
Main Authors: Tom A. P. Driedonks, Maarten K. Nijen Twilhaar, Esther N. M. Nolte-‘t Hoen
Format: Article
Language:English
Published: Taylor & Francis Group 2019-12-01
Series:Journal of Extracellular Vesicles
Subjects:
foetal calf serum
foetal bovine serum
extracellular vesicles
small non-coding rna
in vitro cell culture
culture medium
Online Access:http://dx.doi.org/10.1080/20013078.2018.1552059

Internet

http://dx.doi.org/10.1080/20013078.2018.1552059

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