Nucleosomes impede Cas9 access to DNA in vivo and in vitro

Nucleosomes impede Cas9 access to DNA in vivo and in vitro

The prokaryotic CRISPR (clustered regularly interspaced palindromic repeats)-associated protein, Cas9, has been widely adopted as a tool for editing, imaging, and regulating eukaryotic genomes. However, our understanding of how to select single-guide RNAs (sgRNAs) that mediate efficient Cas9 activit...

Full description

Bibliographic Details
Main Authors: Max A Horlbeck, Lea B Witkowsky, Benjamin Guglielmi, Joseph M Replogle, Luke A Gilbert, Jacqueline E Villalta, Sharon E Torigoe, Robert Tjian, Jonathan S Weissman
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2016-03-01
Series:eLife
Subjects:
CRISPR/Cas
nucleosomes
chromatin
Online Access:https://elifesciences.org/articles/12677

Internet

https://elifesciences.org/articles/12677

Similar Items