Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium. Hence, we have developed an efficient method for purifying CSFV from cell-culture medium. Pure viral particles were o...

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Bibliographic Details
Main Author: Ruining WANG,Yubao ZHI,Junqing GUO,Qingmei LI,Li WANG,Jifei YANG,Qianyue JIN,Yinbiao WANG,Yanyan YANG,Guangxu XING,Songlin QIAO,Mengmeng ZHAO,Ruiguang DENG,Gaiping ZHANG
Format: Article
Language:English
Published: Higher Education Press 2015-09-01
Series:Frontiers of Agricultural Science and Engineering
Subjects:
Online Access:http://academic.hep.com.cn/fase/fileup/2095-7505/PDF/2095-7505-2015-3-230.pdf