Microfluidic Irreversible Electroporation—A Versatile Tool to Extract Intracellular Contents of Bacteria and Yeast

Microfluidic Irreversible Electroporation—A Versatile Tool to Extract Intracellular Contents of Bacteria and Yeast

Exploring the dynamic behavior of cellular metabolism requires a standard laboratory method that guarantees rapid sampling and extraction of the cellular content. We propose a versatile sampling technique applicable to cells with different cell wall and cell membrane properties. The technique is bas...

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Bibliographic Details
Main Authors: Alexander Rockenbach, Suresh Sudarsan, Judith Berens, Michael Kosubek, Jaroslav Lazar, Philipp Demling, René Hanke, Philip Mennicken, Birgitta E. Ebert, Lars M. Blank, Uwe Schnakenberg
Format: Article
Language:English
Published: MDPI AG 2019-09-01
Series:Metabolites
Subjects:
irreversible electroporation
microfluidics
microelectrodes
pulsed electric field electroporation
intracellular metabolites
enzymes
quenching
<i>e. coli</i>
<i>s. cerevisiae</i>
Online Access:https://www.mdpi.com/2218-1989/9/10/211

Internet

https://www.mdpi.com/2218-1989/9/10/211

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