Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

Direct Light-up of cAMP Derivatives in Living Cells by Click Reactions

8-Azidoadenosine 3′,5′-cyclic monophosphate (8-azido cAMP) was directly detected in living cells, by applying Cu-free azide-alkyne cycloaddition to probe cAMP derivatives by fluorescence light-up. Fluorescence emission was generated by two non-fluorescent molecules, 8-azido cAMP as a model target an...

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Bibliographic Details
Main Authors: Yan Xu, Hongshan Liu, Tetsuya Hayashi, Makoto Komiyama, Kenichiro Ito
Format: Article
Language:English
Published: MDPI AG 2013-10-01
Series:Molecules
Subjects:
click chemistry
cAMP
fluorescence
cAMP localization in living cells
Online Access:http://www.mdpi.com/1420-3049/18/10/12909

Internet

http://www.mdpi.com/1420-3049/18/10/12909

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