BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction.

BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction.

Next Generation Sequencing (NGS) is driving rapid advancement in biological understanding and RNA-sequencing (RNA-seq) has become an indispensable tool for biology and medicine. There is a growing need for access to these technologies although preparation of NGS libraries remains a bottleneck to wi...

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Bibliographic Details
Main Authors: Brad Thomas Townsley, Michael F Covington, Yasunori eIchihashi, Kristina eZumstein, Neelima Roy Sinha
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-05-01
Series:Frontiers in Plant Science
Subjects:
NGS
Illumina
Digital gene expression
Adapters
strand-specific RNA-seq
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00366/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00366/full

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