Adventitious Virus Detection in Cells by High-Throughput Sequencing of Newly Synthesized RNAs: Unambiguous Differentiation of Cell Infection from Carryover of Viral Nucleic Acids

Adventitious Virus Detection in Cells by High-Throughput Sequencing of Newly Synthesized RNAs: Unambiguous Differentiation of Cell Infection from Carryover of Viral Nucleic Acids

The use of high-throughput sequencing (HTS) to identify viral contamination of biological products is extremely sensitive and provides a broad range of detection. Nevertheless, viral sequences identified can also be inert. Examples include contamination resulting from reagents or the presence of ina...

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Bibliographic Details
Main Authors: Justine Cheval, Erika Muth, Gaëlle Gonzalez, Muriel Coulpier, Pascale Beurdeley, Stéphane Cruveiller, Marc Eloit
Format: Article
Language:English
Published: American Society for Microbiology 2019-06-01
Series:mSphere
Subjects:
4-thiouridine
RNA-seq
Vero
biologicals
high-throughput sequencing
metagenomics
squirrel monkey retrovirus
tick-borne encephalitis virus
virus safety
Online Access:https://doi.org/10.1128/mSphere.00298-19

Internet

https://doi.org/10.1128/mSphere.00298-19

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