RECOMBINANT FLUORESCENT SENSOR OF HYDROGEN PEROXIDE HyPer FUSED WITH ADAPTOR PROTEIN Ruk/CIN85: DESIGNING OF EXPRESSION VECTOR AND ITS FUNCTIONAL CHARACTERIZATION
The aim of this study was to design the expression vector encoding fluorescent sensor of hydrogen peroxide HyPer fused with adaptor protein Ruk/CIN85 as well as to check its subcellular distribution and ability to sense hydrogen peroxide. It was demonstrated that in transiently transfected HEK293 an...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2015-10-01
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Series: | Biotechnologia Acta |
Subjects: | |
Online Access: | http://biotechnology.kiev.ua/index.php?option=com_content&view=article&id=633%3A-hyper-rukcin85-1-a-2-2-2-&catid=102%3A52015&Itemid=126&lang=en |