RECOMBINANT FLUORESCENT SENSOR OF HYDROGEN PEROXIDE HyPer FUSED WITH ADAPTOR PROTEIN Ruk/CIN85: DESIGNING OF EXPRESSION VECTOR AND ITS FUNCTIONAL CHARACTERIZATION

The aim of this study was to design the expression vector encoding fluorescent sensor of hydrogen peroxide HyPer fused with adaptor protein Ruk/CIN85 as well as to check its subcellular distribution and ability to sense hydrogen peroxide. It was demonstrated that in transiently transfected HEK293 an...

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Bibliographic Details
Main Authors: А. V. Bazalii, A. V. Vorotnikov, P. A. Тiurin-Кuzmin, V. А. Тkachuk, S. V. Коmisarenko, L. B. Drobot
Format: Article
Language:English
Published: National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. 2015-10-01
Series:Biotechnologia Acta
Subjects:
Online Access:http://biotechnology.kiev.ua/index.php?option=com_content&view=article&id=633%3A-hyper-rukcin85-1-a-2-2-2-&catid=102%3A52015&Itemid=126&lang=en