Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3

The Class 2 family of CRISPR nucleases named Cas12j, which shares only low sequence identity with other CRISPR nucleases was recently identified in the biggiephage clade of phages. Here, the authors present the cryo-EM structure of a functional Cas12j3−crRNA complex in the post-catalytic state and d...

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Bibliographic Details
Main Authors: Arturo Carabias, Anders Fuglsang, Piero Temperini, Tillmann Pape, Nicholas Sofos, Stefano Stella, Simon Erlendsson, Guillermo Montoya
Format: Article
Language:English
Published: Nature Publishing Group 2021-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-24707-3