Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors
Summary Introduction: Breast cancer is the most cause of death, and approximately 90% of these deaths are due to metastases. Matrix metalloproteinase-2 (MMP-2) gelatinase activity is able to degrade a major constituent of the tumor microenvironment, type IV collagen. Two well-established proteins u...
Main Authors: | , , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Associação Médica Brasileira
|
Series: | Revista da Associação Médica Brasileira |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302016000800774&lng=en&tlng=en |
id |
doaj-0c6efe47b2694a2e8f9744737daed40a |
---|---|
record_format |
Article |
spelling |
doaj-0c6efe47b2694a2e8f9744737daed40a2020-11-24T23:38:29ZengAssociação Médica BrasileiraRevista da Associação Médica Brasileira1806-928262877478110.1590/1806-9282.62.08.774S0104-42302016000800774Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptorsEdneia A. S. RamosCamila T. da SilvaGraciele C. M. ManicaIsabela T. PereiraLiliane M. B. KlassenEnilze M. S. F. RibeiroIglenir J. CavalliKarin Braun-PradoRubens S. LimaCicero A. UrbanFabrício F. CostaLucia de NoronhaGiseli KlassenSummary Introduction: Breast cancer is the most cause of death, and approximately 90% of these deaths are due to metastases. Matrix metalloproteinase-2 (MMP-2) gelatinase activity is able to degrade a major constituent of the tumor microenvironment, type IV collagen. Two well-established proteins used as markers in clinical practice for breast cancer are the receptors for estrogen (ER) and progesterone (PR). Although the presence of these receptors has been associated with a better prognosis, loss of these proteins can occur during tumor progression, with subsequent resistance to hormone therapy. Objective: To study the correlation among MMP-2, ER, and PR, as well as the establishment of the metastatic process in primary breast tumors. Method: Breast cancer samples (n=44) were analyzed by immunohistochemistry for MMP-2, ER, and PR. Results: We observed that 90% of patients who had metastases and died showed positive staining for MMP-2 (p=0.0082 for both). Using Kaplan-Meier analysis, we found that negative ER patients who were also positive for MMP-2 had even worse disease-free survival (DFS) and overall survival (OS) (p= 0.012 and p=0.005, respectively). Similar results were found in PR-negative patients for DFS (a trend p=0.077) and OS (p=0.038). Conclusion: Regardless of our small sample size (n=44), the data obtained strongly suggest that MMP-2 in combination with already well-established markers could help to predict the emergence of metastases and death in patients with breast cancer.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302016000800774&lng=en&tlng=enneoplasias da mamaMMP-2imuno-histoquímicamarcador molecular |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Edneia A. S. Ramos Camila T. da Silva Graciele C. M. Manica Isabela T. Pereira Liliane M. B. Klassen Enilze M. S. F. Ribeiro Iglenir J. Cavalli Karin Braun-Prado Rubens S. Lima Cicero A. Urban Fabrício F. Costa Lucia de Noronha Giseli Klassen |
spellingShingle |
Edneia A. S. Ramos Camila T. da Silva Graciele C. M. Manica Isabela T. Pereira Liliane M. B. Klassen Enilze M. S. F. Ribeiro Iglenir J. Cavalli Karin Braun-Prado Rubens S. Lima Cicero A. Urban Fabrício F. Costa Lucia de Noronha Giseli Klassen Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors Revista da Associação Médica Brasileira neoplasias da mama MMP-2 imuno-histoquímica marcador molecular |
author_facet |
Edneia A. S. Ramos Camila T. da Silva Graciele C. M. Manica Isabela T. Pereira Liliane M. B. Klassen Enilze M. S. F. Ribeiro Iglenir J. Cavalli Karin Braun-Prado Rubens S. Lima Cicero A. Urban Fabrício F. Costa Lucia de Noronha Giseli Klassen |
author_sort |
Edneia A. S. Ramos |
title |
Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors |
title_short |
Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors |
title_full |
Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors |
title_fullStr |
Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors |
title_full_unstemmed |
Worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive MMP-2 and negative estrogen and progesterone receptors |
title_sort |
worse prognosis in breast cancer patients can be predicted by immunohistochemical analysis of positive mmp-2 and negative estrogen and progesterone receptors |
publisher |
Associação Médica Brasileira |
series |
Revista da Associação Médica Brasileira |
issn |
1806-9282 |
description |
Summary Introduction: Breast cancer is the most cause of death, and approximately 90% of these deaths are due to metastases. Matrix metalloproteinase-2 (MMP-2) gelatinase activity is able to degrade a major constituent of the tumor microenvironment, type IV collagen. Two well-established proteins used as markers in clinical practice for breast cancer are the receptors for estrogen (ER) and progesterone (PR). Although the presence of these receptors has been associated with a better prognosis, loss of these proteins can occur during tumor progression, with subsequent resistance to hormone therapy. Objective: To study the correlation among MMP-2, ER, and PR, as well as the establishment of the metastatic process in primary breast tumors. Method: Breast cancer samples (n=44) were analyzed by immunohistochemistry for MMP-2, ER, and PR. Results: We observed that 90% of patients who had metastases and died showed positive staining for MMP-2 (p=0.0082 for both). Using Kaplan-Meier analysis, we found that negative ER patients who were also positive for MMP-2 had even worse disease-free survival (DFS) and overall survival (OS) (p= 0.012 and p=0.005, respectively). Similar results were found in PR-negative patients for DFS (a trend p=0.077) and OS (p=0.038). Conclusion: Regardless of our small sample size (n=44), the data obtained strongly suggest that MMP-2 in combination with already well-established markers could help to predict the emergence of metastases and death in patients with breast cancer. |
topic |
neoplasias da mama MMP-2 imuno-histoquímica marcador molecular |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302016000800774&lng=en&tlng=en |
work_keys_str_mv |
AT edneiaasramos worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT camilatdasilva worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT gracielecmmanica worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT isabelatpereira worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT lilianembklassen worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT enilzemsfribeiro worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT iglenirjcavalli worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT karinbraunprado worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT rubensslima worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT ciceroaurban worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT fabriciofcosta worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT luciadenoronha worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors AT giseliklassen worseprognosisinbreastcancerpatientscanbepredictedbyimmunohistochemicalanalysisofpositivemmp2andnegativeestrogenandprogesteronereceptors |
_version_ |
1725516903788576768 |