Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Expression of in vivo biotinylated recombinant antigens SAG1 and SAG2A from Toxoplasma gondii for improved seroepidemiological bead-based multiplex assays

Abstract Background Few bead-based multiplex assays have been described that detect antibodies against the protozoan parasite Toxoplasma gondii in large-scale seroepidemiological surveys. Moreover, each multiplex assay has specific variations or limitations, such as the use of truncated or fusion pr...

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Bibliographic Details
Main Authors: Sandra Klein, Daniel Stern, Frank Seeber
Format: Article
Language:English
Published: BMC 2020-10-01
Series:BMC Biotechnology
Subjects:
Toxoplasma gondii
Surface antigens
Bead-based multiplex assay
Biotinylation tag
Diagnosis
Seroepidemiology
Online Access:http://link.springer.com/article/10.1186/s12896-020-00646-7

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http://link.springer.com/article/10.1186/s12896-020-00646-7

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