NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release
NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10−/− mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10−/− dendritic cells (DCs)...
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Format: | Article |
Language: | English |
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Frontiers Media S.A.
2017-11-01
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Series: | Frontiers in Immunology |
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Online Access: | http://journal.frontiersin.org/article/10.3389/fimmu.2017.01462/full |
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record_format |
Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maurizio Vacca Maurizio Vacca Julia Böhme Lia Paola Zambetti Hanif Javanmard Khameneh Bhairav S. Paleja Federica Laudisi Adrian W. S. Ho Kurt Neo Keith Weng Kit Leong Mardiana Marzuki Bernett Lee Michael Poidinger Laura Santambrogio Liana Tsenova Francesca Zolezzi Gennaro De Libero Gennaro De Libero Amit Singhal Amit Singhal Alessandra Mortellaro Alessandra Mortellaro |
spellingShingle |
Maurizio Vacca Maurizio Vacca Julia Böhme Lia Paola Zambetti Hanif Javanmard Khameneh Bhairav S. Paleja Federica Laudisi Adrian W. S. Ho Kurt Neo Keith Weng Kit Leong Mardiana Marzuki Bernett Lee Michael Poidinger Laura Santambrogio Liana Tsenova Francesca Zolezzi Gennaro De Libero Gennaro De Libero Amit Singhal Amit Singhal Alessandra Mortellaro Alessandra Mortellaro NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release Frontiers in Immunology NLRP10 dendritic cells CpG DNA toll-like receptor 9 IL-12 T helper 1 |
author_facet |
Maurizio Vacca Maurizio Vacca Julia Böhme Lia Paola Zambetti Hanif Javanmard Khameneh Bhairav S. Paleja Federica Laudisi Adrian W. S. Ho Kurt Neo Keith Weng Kit Leong Mardiana Marzuki Bernett Lee Michael Poidinger Laura Santambrogio Liana Tsenova Francesca Zolezzi Gennaro De Libero Gennaro De Libero Amit Singhal Amit Singhal Alessandra Mortellaro Alessandra Mortellaro |
author_sort |
Maurizio Vacca |
title |
NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release |
title_short |
NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release |
title_full |
NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release |
title_fullStr |
NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release |
title_full_unstemmed |
NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 Release |
title_sort |
nlrp10 enhances cd4+ t-cell-mediated ifnγ response via regulation of dendritic cell-derived il-12 release |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-11-01 |
description |
NLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10−/− mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10−/− dendritic cells (DCs) elicited sub-optimal IFNγ production by antigen-specific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10−/− DCs produced low levels of IL-12, due to a substantial decrease in NF-κB activation. Defective IL-12 production was also evident in vivo and affected IFNγ production by CD4+ T cells. Upon Mycobacterium tuberculosis (Mtb) infection, Nlrp10−/− mice displayed diminished T helper 1-cell responses and increased bacterial growth compared to WT mice. These data indicate that NLRP10-mediated IL-12 production by DCs is critical for IFNγ induction in T cells and contributes to promote the host defense against Mtb. |
topic |
NLRP10 dendritic cells CpG DNA toll-like receptor 9 IL-12 T helper 1 |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.01462/full |
work_keys_str_mv |
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doaj-0d658a4472974b4ead4b0eed71d2a1e92020-11-25T00:02:13ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-11-01810.3389/fimmu.2017.01462296354NLRP10 Enhances CD4+ T-Cell-Mediated IFNγ Response via Regulation of Dendritic Cell-Derived IL-12 ReleaseMaurizio Vacca0Maurizio Vacca1Julia Böhme2Lia Paola Zambetti3Hanif Javanmard Khameneh4Bhairav S. Paleja5Federica Laudisi6Adrian W. S. Ho7Kurt Neo8Keith Weng Kit Leong9Mardiana Marzuki10Bernett Lee11Michael Poidinger12Laura Santambrogio13Liana Tsenova14Francesca Zolezzi15Gennaro De Libero16Gennaro De Libero17Amit Singhal18Amit Singhal19Alessandra Mortellaro20Alessandra Mortellaro21Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeDepartment of Pathology, Albert Einstein College of Medicine, New York, NY, United StatesDepartment of Biological Sciences, New York City College of Technology, Brooklyn, NY, United StatesSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeDepartment of Biomedicine, University of Basel and University Hospital Basel, Basel, SwitzerlandSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeLee Kong Chian School of Medicine, Nanyang Technological University, Singapore, SingaporeSingapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, SingaporeDepartment of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SingaporeNLRP10 is a nucleotide-binding oligomerization domain-like receptor that functions as an intracellular pattern recognition receptor for microbial products. Here, we generated a Nlrp10−/− mouse to delineate the role of NLRP10 in the host immune response and found that Nlrp10−/− dendritic cells (DCs) elicited sub-optimal IFNγ production by antigen-specific CD4+ T cells compared to wild-type (WT) DCs. In response to T-cell encounter, CD40 ligation or Toll-like receptor 9 stimulation, Nlrp10−/− DCs produced low levels of IL-12, due to a substantial decrease in NF-κB activation. Defective IL-12 production was also evident in vivo and affected IFNγ production by CD4+ T cells. Upon Mycobacterium tuberculosis (Mtb) infection, Nlrp10−/− mice displayed diminished T helper 1-cell responses and increased bacterial growth compared to WT mice. These data indicate that NLRP10-mediated IL-12 production by DCs is critical for IFNγ induction in T cells and contributes to promote the host defense against Mtb.http://journal.frontiersin.org/article/10.3389/fimmu.2017.01462/fullNLRP10dendritic cellsCpG DNAtoll-like receptor 9IL-12T helper 1 |