Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP

Genetic transformation of Drosophila willistoni using piggyBac transposon and GFP

Studies were carried out on the use of piggyBac transposable element as vector and the green fluorescent protein (EGFP) from the jellyfish, Aquorea victoria, as a genetic marker for the transformation of Drosophila willistoni. Preblastoderm embryos of D. willistoni white mutant were microinjected wi...

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Bibliographic Details
Main Authors: Manuela Finokiet, Beatriz Goni, Élgion Lúcio Silva Loreto
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2007-01-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Transposable elements
insect transgenesis
germ-line transformation
fluorescent protein marker
microinjection
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100013

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