Laboratory-Scale Isolation of Insect Antifreeze Protein for Cryobiology

Laboratory-Scale Isolation of Insect Antifreeze Protein for Cryobiology

Micromolar concentrations of hyperactive antifreeze proteins (AFPs) from insects can prevent aqueous solutions from freezing down to at least −6 °C. To explore cryopreservation of cells, tissues and organs at these temperatures without ice formation, we have developed a protocol t...

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Bibliographic Details
Main Authors: Heather E. Tomalty, Laurie A. Graham, Robert Eves, Audrey K. Gruneberg, Peter L. Davies
Format: Article
Language:English
Published: MDPI AG 2019-05-01
Series:Biomolecules
Subjects:
antifreeze protein
ice-affinity purification
thermal hysteresis
cryopreservation
Online Access:https://www.mdpi.com/2218-273X/9/5/180

Internet

https://www.mdpi.com/2218-273X/9/5/180

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