Optimization of Neutral Comet Assay for studying DNA double-strand breaks in pea and wheat

This study describes an adaptation of the Comet assay under neutral conditions for mono- and dicotyledonous plants pea (Pisum sativum L.) and wheat (Triticum aestivum L.). Modifications concern lysis and electrophoresis steps, respectively. Electrophoresis was carried out varying the intensity of th...

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Bibliographic Details
Main Authors: Ivelina Nikolova, Mariyana Georgieva, Lubomir Stoilov, Zornica Katerova, Desisslava Todorova
Format: Article
Language:English
Published: Plovdiv University Press 2013-01-01
Series:Journal of BioScience and Biotechnology
Subjects:
UVC
Online Access:http://www.jbb.uni-plovdiv.bg/documents/27807/59547/jbb_2013-2(3)-pages_151-157.pdf