A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh+, tdh+, and ureR+)

A Novel qPCR Method for Simultaneous Detection and Quantification of Viable Pathogenic and Non-pathogenic Vibrio parahaemolyticus (tlh+, tdh+, and ureR+)

Pathogenic and non-pathogenic Vibrio parahaemolyticus strains were simultaneously detected and quantified using a novel viable multiplex real-time PCR (novel qPCR). We used a new PCR primer and probe, ureR, as a surrogate for detection of the toxin trh gene as the primer was better at identifying va...

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Bibliographic Details
Main Authors: Ben Niu, Bin Hong, Zhaohuan Zhang, Lili Mu, Pradeep K. Malakar, Haiquan Liu, Yingjie Pan, Yong Zhao
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-08-01
Series:Frontiers in Microbiology
Subjects:
novel qPCR
PMA
pathogenic and non-pathogenic V. parahaemolyticus
rapid detection technique
shrimp
clam
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2018.01747/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2018.01747/full

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