Expression of the melittin gene of Apis cerana cerana (Hymenoptera: Apidae) in insect cells
A fragment encoding melittin cDNA from Apis cerana cerana fused with glutathione S-transferase gene was inserted into the multiple cloning site of the pBacFastHTb to construct a recombinant donor plasmid, pBacHT-GSTAccM, which was transposed to the target bacmid in E. coli (DH10) by Tn7 transpositio...
Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Institute of Entomology, Biology Centre, Czech Academy of Science
2006-10-01
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Series: | European Journal of Entomology |
Subjects: | |
Online Access: | https://www.eje.cz/artkey/eje-200604-0024_Expression_of_the_melittin_gene_of_Apis_cerana_cerana_Hymenoptera_Apidae_in_insect_cells.php |