Expression of the melittin gene of Apis cerana cerana (Hymenoptera: Apidae) in insect cells

A fragment encoding melittin cDNA from Apis cerana cerana fused with glutathione S-transferase gene was inserted into the multiple cloning site of the pBacFastHTb to construct a recombinant donor plasmid, pBacHT-GSTAccM, which was transposed to the target bacmid in E. coli (DH10) by Tn7 transpositio...

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Bibliographic Details
Main Authors: Wan-Jun SHI, Jia-An CHENG, Chuan-Xi ZHANG
Format: Article
Language:English
Published: Institute of Entomology, Biology Centre, Czech Academy of Science 2006-10-01
Series:European Journal of Entomology
Subjects:
Online Access:https://www.eje.cz/artkey/eje-200604-0024_Expression_of_the_melittin_gene_of_Apis_cerana_cerana_Hymenoptera_Apidae_in_insect_cells.php