A semi-nested PCR assay for molecular detection of Paracoccidioides brasiliensis in tissue samples Semi-nested PCR para a detecção molecular de Paracoccidioides brasiliensis em amostras de tecido

INTRODUCTION: Paracoccidioidomycosis is a systemic infection caused by Paracoccidioides brasiliensis. METHODS: In this study, a semi-nested PCR for paracoccidioidomycosis diagnosis was developed. The primers ITS1 and ITS4 were used in the first reaction, while the primers MJ03 and ITS1 primer were u...

Full description

Bibliographic Details
Main Authors: Andrea Cristine Koishi, Débora Fonseca Vituri, Pedro Sebastião Raimundo Dionízio Filho, Alexandre Augusto Sasaki, Maria Sueli Soares Felipe, Emerson José Venancio
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2010-12-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822010000600026