Analysis of cellulose synthase activity in Arabidopsis using spinning disk microscopy

Summary: We describe sample preparation and visualization of fluorescently tagged cellulose synthases in cellulose synthase complexes at the plasma membrane of Arabidopsis hypocotyl epidermal cells using live-cell imaging via spinning disk microscopy. We present a technique for sample mounting that...

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Bibliographic Details
Main Authors: Jana Verbančič, Jenny Jiahui Huang, Heather E. McFarlane
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005694