Quantitative spectrofluorometric assay detecting nuclear condensation and fragmentation in intact cells

Abstract At present, nuclear condensation and fragmentation have been estimated also using Hoechst probes in fluorescence microscopy and flow cytometry. However, none of the methods used the Hoechst probes for quantitative spectrofluorometric assessment. Therefore, the aim of the present study was t...

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Bibliographic Details
Main Authors: Majtnerova Pavlina, Capek Jan, Petira Filip, Handl Jiri, Rousar Tomas
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-91380-3