Optimization of an Activity Assay of Coprinus Cinereus Peroxidase

ABSTRACT To seek a simple, rapid and sensitive Coprinus cinereus Peroxidase (CIP) activity assay, a convenient one-factor-at-a-time (OFAT) method and a response surface methodology (RSM) were used. The recombinant CIP expressed in Pichia pastoris was purified with the Ni-NTA spin column. Based on th...

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Bibliographic Details
Main Authors: Bing Xue Dong, Yu Hong Zhang, Gang Li, Jin Tao Fang, Ji Huan Zheng, Run Qian Mao
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2018-11-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100421&lng=en&tlng=en