Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women

Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women

Abstract Background Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. Methods Genomic DNA (gDNA) was purified from 12 ATCC...

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Bibliographic Details
Main Authors: Daniel F. Escobar, Diego A. Diaz-Dinamarca, Carlos F. Hernández, Daniel A. Soto, Ricardo A. Manzo, Pedro I. Alarcón, Camila H. Pinto, Diego N. Bastias, Carolayn N. Oberg-Bravo, Robert Rojas, Sebastián E. Illanes, Alexis M. Kalergis, Abel E. Vasquez
Format: Article
Language:English
Published: BMC 2020-06-01
Series:BMC Pregnancy and Childbirth
Subjects:
Group B Streptococcus
qPCR
Surface immunogenic protein
Analytical validation
Bacterial detection
Online Access:http://link.springer.com/article/10.1186/s12884-020-03038-z

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http://link.springer.com/article/10.1186/s12884-020-03038-z

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