Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen
Inhibition of fatty acid amide hydrolase (FAAH) reduces the gastrointestinal damage produced by non-steroidal anti-inflammatory agents such as sulindac and indomethacin in experimental animals, suggesting that a dual-action FAAH-cyclooxygenase (COX) inhibitor could have useful therapeutic properties...
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Online Access: | http://dx.doi.org/10.1080/14756366.2020.1743283 |
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doaj-0f50faf3cf0e43df86dcdb218f2f2fdd2021-07-15T13:10:32ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742020-01-0135181582310.1080/14756366.2020.17432831743283Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofenAlessandro Deplano0Jessica Karlsson1Mona Svensson2Federica Moraca3Bruno Catalanotti4Christopher J. Fowler5Valentina Onnis6Unit of Pharmaceutical, Pharmacological and Nutraceutical Sciences, Department of Life and Environmental Sciences, University of CagliariDepartment of Integrative Medical Biology, Umeå UniversityDepartment of Integrative Medical Biology, Umeå UniversityDepartment of Pharmacy, University of Napoli Federico IIDepartment of Pharmacy, University of Napoli Federico IIDepartment of Integrative Medical Biology, Umeå UniversityUnit of Pharmaceutical, Pharmacological and Nutraceutical Sciences, Department of Life and Environmental Sciences, University of CagliariInhibition of fatty acid amide hydrolase (FAAH) reduces the gastrointestinal damage produced by non-steroidal anti-inflammatory agents such as sulindac and indomethacin in experimental animals, suggesting that a dual-action FAAH-cyclooxygenase (COX) inhibitor could have useful therapeutic properties. Here, we have investigated 12 novel amide analogues of ibuprofen as potential dual-action FAAH/COX inhibitors. N-(3-Bromopyridin-2-yl)−2-(4-isobutylphenyl)propanamide (Ibu-AM68) was found to inhibit the hydrolysis of [3H]anandamide by rat brain homogenates by a reversible, mixed-type mechanism of inhibition with a Ki value of 0.26 µM and an α value of 4.9. At a concentration of 10 µM, the compound did not inhibit the cyclooxygenation of arachidonic acid by either ovine COX-1 or human recombinant COX-2. However, this concentration of Ibu-AM68 greatly reduced the ability of the COX-2 to catalyse the cyclooxygenation of the endocannabinoid 2-arachidonoylglycerol. It is concluded that Ibu-AM68 is a dual-acting FAAH/substrate-selective COX inhibitor.http://dx.doi.org/10.1080/14756366.2020.1743283ibuprofen amidesfaah inhibitionfatty acid amide hydrolaseendocannabinoidcyclooxygenase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alessandro Deplano Jessica Karlsson Mona Svensson Federica Moraca Bruno Catalanotti Christopher J. Fowler Valentina Onnis |
spellingShingle |
Alessandro Deplano Jessica Karlsson Mona Svensson Federica Moraca Bruno Catalanotti Christopher J. Fowler Valentina Onnis Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen Journal of Enzyme Inhibition and Medicinal Chemistry ibuprofen amides faah inhibition fatty acid amide hydrolase endocannabinoid cyclooxygenase |
author_facet |
Alessandro Deplano Jessica Karlsson Mona Svensson Federica Moraca Bruno Catalanotti Christopher J. Fowler Valentina Onnis |
author_sort |
Alessandro Deplano |
title |
Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen |
title_short |
Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen |
title_full |
Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen |
title_fullStr |
Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen |
title_full_unstemmed |
Exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen |
title_sort |
exploring the fatty acid amide hydrolase and cyclooxygenase inhibitory properties of novel amide derivatives of ibuprofen |
publisher |
Taylor & Francis Group |
series |
Journal of Enzyme Inhibition and Medicinal Chemistry |
issn |
1475-6366 1475-6374 |
publishDate |
2020-01-01 |
description |
Inhibition of fatty acid amide hydrolase (FAAH) reduces the gastrointestinal damage produced by non-steroidal anti-inflammatory agents such as sulindac and indomethacin in experimental animals, suggesting that a dual-action FAAH-cyclooxygenase (COX) inhibitor could have useful therapeutic properties. Here, we have investigated 12 novel amide analogues of ibuprofen as potential dual-action FAAH/COX inhibitors. N-(3-Bromopyridin-2-yl)−2-(4-isobutylphenyl)propanamide (Ibu-AM68) was found to inhibit the hydrolysis of [3H]anandamide by rat brain homogenates by a reversible, mixed-type mechanism of inhibition with a Ki value of 0.26 µM and an α value of 4.9. At a concentration of 10 µM, the compound did not inhibit the cyclooxygenation of arachidonic acid by either ovine COX-1 or human recombinant COX-2. However, this concentration of Ibu-AM68 greatly reduced the ability of the COX-2 to catalyse the cyclooxygenation of the endocannabinoid 2-arachidonoylglycerol. It is concluded that Ibu-AM68 is a dual-acting FAAH/substrate-selective COX inhibitor. |
topic |
ibuprofen amides faah inhibition fatty acid amide hydrolase endocannabinoid cyclooxygenase |
url |
http://dx.doi.org/10.1080/14756366.2020.1743283 |
work_keys_str_mv |
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1721300877465092096 |