A simple method to increase the proportion of bone marrow-derived macrophages positive for M-CSFR using the reducing agent dithiothreitol (DTT)

Only a few bone marrow-derived macrophages (BM-MΦ) are positive for macrophage colony-stimulating factor receptor (M-CSFR). Thus, a method is needed to increase the proportion of BM-MΦ that are positive for M-CSFR to facilitate the investigation of the effects of M-CSFR downregulation on various dis...

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Bibliographic Details
Main Authors: Ryota Hashimoto, Hiroyuki Daida, Takao Okada, Youichi Katoh
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:MethodsX
Online Access:http://www.sciencedirect.com/science/article/pii/S221501611830195X