High throughput single-cell detection of multiplex CRISPR-edited gene modifications

High throughput single-cell detection of multiplex CRISPR-edited gene modifications

Abstract CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells en...

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Bibliographic Details
Main Authors: Elisa ten Hacken, Kendell Clement, Shuqiang Li, María Hernández-Sánchez, Robert Redd, Shu Wang, David Ruff, Michaela Gruber, Kaitlyn Baranowski, Jose Jacob, James Flynn, Keith W. Jones, Donna Neuberg, Kenneth J. Livak, Luca Pinello, Catherine J. Wu
Format: Article
Language:English
Published: BMC 2020-10-01
Series:Genome Biology
Subjects:
Genetics
Single cell
Genome editing
Loss-of-function
Mutation
CRISPR-Cas9
Online Access:http://link.springer.com/article/10.1186/s13059-020-02174-1

Internet

http://link.springer.com/article/10.1186/s13059-020-02174-1

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