Standardizing electrophoresis conditions: how to eliminate a major source of error in the comet assay.
In the alkaline comet assay, cells are embedded in agarose, lysed, and then subjected to further processing including electrophoresis at high pH (>13). We observed very large variations of mean comet tail lengths of cell samples from the same population when spread on a glass or plastic substrate...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-06-01
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| Series: | Frontiers in Genetics |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/conf.fgene.2015.01.00048/full |