Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays

Abstract Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate a specific luciferase with unique luc...

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Bibliographic Details
Main Authors: Ryo Nishihara, Masahiro Abe, Shigeru Nishiyama, Daniel Citterio, Koji Suzuki, Sung Bae Kim
Format: Article
Language:English
Published: Nature Publishing Group 2017-04-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-017-00955-6