A fluorescence anisotropy assay to discover and characterize ligands targeting the maytansine site of tubulin
Microtubule-targeting agents are used successfully as anticancer therapeutics. Here authors develop a fluorescence-anisotropy-based assay to identify and characterize ligands for the maytansine site of tubulin and provide crystal structures of identified ligands in complex with tubulin.
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Publishing Group
2018-05-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-018-04535-8 |