Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)

Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in rout...

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Bibliographic Details
Main Authors: Amin Forootan, Robert Sjöback, Jens Björkman, Björn Sjögreen, Lucas Linz, Mikael Kubista
Format: Article
Language:English
Published: Elsevier 2017-06-01
Series:Biomolecular Detection and Quantification
Subjects:
LoD
LoQ
Online Access:http://www.sciencedirect.com/science/article/pii/S2214753516300286