An Immunofluorescence-assisted Microfluidic Single Cell Quantitative Reverse Transcription Polymerase Chain Reaction Analysis of Tumour Cells Separated from Blood

An Immunofluorescence-assisted Microfluidic Single Cell Quantitative Reverse Transcription Polymerase Chain Reaction Analysis of Tumour Cells Separated from Blood

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Circulating tumour cells (CTCs) are important indicators of metastatic cancer and may provide critical information for individualized treatment. As CTCs are usually very rare, the techniques to obtain information from very small numbers of cells are crucial. Here, we propose a method to perform a si...

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Bibliographic Details
Main Authors: Kazunori Hoshino, HaeWon Chung, Chun-Hsien Wu, Kaarthik Rajendran, Yu-Yen Huang, Peng Chen, Konstantin V. Sokolov, Jonghwan Kim, John X.J. Zhang
Format: Article
Language:English
Published: AboutScience Srl 2015-11-01
Series:Journal of Circulating Biomarkers
Subjects:
CTC
single cell PCR
breast cancer
immunomagnetic assay
lab on a chip
laser microdissection
Online Access:http://www.intechopen.com/journals/journal-of-circulating-biomarkers/an-immunofluorescence-assisted-microfluidic-single-cell-quantitative-reverse-transcription-polymeras

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http://www.intechopen.com/journals/journal-of-circulating-biomarkers/an-immunofluorescence-assisted-microfluidic-single-cell-quantitative-reverse-transcription-polymeras

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