Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation.
We have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was met...
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2010-11-01
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doaj-106999c7f7154d9dbec1d571f844a5a92020-11-25T01:51:08ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-11-01511e1384010.1371/journal.pone.0013840Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation.Francesca LessiAndrew BeggsMariagrazia de PaloMarcello AntiRaffaele Macarone PalmieriSimona FrancesconiVito GomesGeneroso BevilacquaIan TomlinsonStefania SegditsasWe have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was methylated in colonic tumour samples.We investigated the methylation profile of the two CpG islands present in the promoter region of SGK1 in a panel of 5 colorectal cancer cell lines by sequencing clones of bisulphite-treated DNA samples. We further confirmed our findings in a panel of 10 normal and 10 tumour colonic tissue samples of human origin. We observed CpG methylation only in the smaller and more distal CpG island in the promoter region of SGK1 in both normal and tumour samples of colonic origin. We further identified a single nucleotide polymorphism (SNP, rs1743963) which affects methylation of the corresponding CpG.Our results show that even though partial methylation of the promoter region of SGK1 is present, this does not account for the different expression levels seen between normal and tumour tissue.http://europepmc.org/articles/PMC2974649?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Francesca Lessi Andrew Beggs Mariagrazia de Palo Marcello Anti Raffaele Macarone Palmieri Simona Francesconi Vito Gomes Generoso Bevilacqua Ian Tomlinson Stefania Segditsas |
spellingShingle |
Francesca Lessi Andrew Beggs Mariagrazia de Palo Marcello Anti Raffaele Macarone Palmieri Simona Francesconi Vito Gomes Generoso Bevilacqua Ian Tomlinson Stefania Segditsas Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. PLoS ONE |
author_facet |
Francesca Lessi Andrew Beggs Mariagrazia de Palo Marcello Anti Raffaele Macarone Palmieri Simona Francesconi Vito Gomes Generoso Bevilacqua Ian Tomlinson Stefania Segditsas |
author_sort |
Francesca Lessi |
title |
Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. |
title_short |
Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. |
title_full |
Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. |
title_fullStr |
Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. |
title_full_unstemmed |
Down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. |
title_sort |
down-regulation of serum/glucocorticoid regulated kinase 1 in colorectal tumours is largely independent of promoter hypermethylation. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2010-11-01 |
description |
We have previously shown that serum/glucocorticoid regulated kinase 1 (SGK1) is down-regulated in colorectal cancers (CRC) with respect to normal tissue. As hyper-methylation of promoter regions is a well-known mechanism of gene silencing in cancer, we tested whether the SGK1 promoter region was methylated in colonic tumour samples.We investigated the methylation profile of the two CpG islands present in the promoter region of SGK1 in a panel of 5 colorectal cancer cell lines by sequencing clones of bisulphite-treated DNA samples. We further confirmed our findings in a panel of 10 normal and 10 tumour colonic tissue samples of human origin. We observed CpG methylation only in the smaller and more distal CpG island in the promoter region of SGK1 in both normal and tumour samples of colonic origin. We further identified a single nucleotide polymorphism (SNP, rs1743963) which affects methylation of the corresponding CpG.Our results show that even though partial methylation of the promoter region of SGK1 is present, this does not account for the different expression levels seen between normal and tumour tissue. |
url |
http://europepmc.org/articles/PMC2974649?pdf=render |
work_keys_str_mv |
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